RmcB (ATCC® CRL-2379)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The RmcB cell line was established in 1985 by R. L. Crowell.
The antibody reacts with human CAR (the receptor for coxsackie B virus and the binding protein for adenovirus 2 and 3).
Derivation
A/J mice were immunized with a 50-kilodalton receptor protein (Rp-a) for the group B coxsackieviruses (CB) isolated in a virus-receptor complex from detergent-solubilized HeLa cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The RmcB cell line was established in 1985 by R. L. Crowell.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CAR (Coxsackie - Adenovirus Receptor)
Cellular Products
immunoglobulin; monoclonal antibody; against human CAR (Coxsackie - Adenovirus Receptor)
Comments
A/J mice were immunized with a 50-kilodalton receptor protein (Rp-a) for the group B coxsackieviruses (CB) isolated in a virus-receptor complex from detergent-solubilized HeLa cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The RmcB cell line was established in 1985 by R. L. Crowell.
The antibody reacts with human CAR (the receptor for coxsackie B virus and the binding protein for adenovirus 2 and 3).
The antibody can block binding of adenovirus and block binding and infection by coxsackie B Virus.
Mouse monoclonal antibody (RmcB) protects HeLa cells and Buffalo green monkey kidney cells from infection by all six serotypes of CB. RmcB did not protect HeLa cells from infection by poliovirus, echovirus 6, or coxsackievirus A18.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cultures between 1 X 10 exp5 and 9 X 10 exp5 cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor RW Finberg
Year of Origin 1985
References

Bergelson JM, et al. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science 275: 1320-1323, 1997. PubMed: 9036860

Mapoles JE, et al. Purification of a HeLa cell receptor protein for group B coxsackieviruses. J. Virol. 55: 560-566, 1985. PubMed: 2991580

Hsu KH, et al. A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses. J. Virol. 62: 1647-1652, 1988. PubMed: 2451756

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Bergelson JM, et al. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science 275: 1320-1323, 1997. PubMed: 9036860

Mapoles JE, et al. Purification of a HeLa cell receptor protein for group B coxsackieviruses. J. Virol. 55: 560-566, 1985. PubMed: 2991580

Hsu KH, et al. A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses. J. Virol. 62: 1647-1652, 1988. PubMed: 2451756