LA7 (ATCC® CRL-2283)

Organism: Rattus norvegicus, rat  /  Cell Type: Epithelial  /  Tissue: mammary gland  / 

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Organism Rattus norvegicus, rat
Tissue mammary gland
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Strain Sprague-Dawley
Applications
LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA.
Cells irradiated with 6000 rads may be used as feeder layers.
Storage Conditions liquid nitrogen vapor phase
Derivation
LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA.
It is a clonal derivative of the Rama 25 line obtained from a rat treated with dimethylbenz(a)anthracene.
Comments
LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA.
It is a clonal derivative of the Rama 25 line obtained from a rat treated with dimethylbenz(a)anthracene.
Cells irradiated with 6000 rads may be used as feeder layers.
Such feeder layers will stimulate the proliferation of normal primary mouse and rat mammary cells and normal primary mouse thymic epithelial cells.
Complete Growth Medium Dulbecco's modified Eagle's medium with reduced sodium bicarbonate (1.5 to 2.0 g/L), 4.5 g/L glucose, 0.005 mg/ml insulin, 50 nM hydrocortisone and 20 mM HEPES, 95%; fetal bovine serum, 5%


Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (RefBerthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212). The Phenol Red-free version of DMEM, is ATCC catalog No. 30-2601.

Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:8 to 1:10
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor UK Ehmann
Year of Origin 1979
References

Ehmann UK, et al. To grow mouse mammary epithelial cells in culture. J. Cell Biol. 98: 1026-1032, 1984. PubMed: 6699079

Dulbecco R, et al. Differentiation of a rat mammary cell line in vitro. Proc. Natl. Acad. Sci. USA 76: 1256-1260, 1979. PubMed: 375233

Ehmann UK, et al. Long-term proliferation of mouse thymic epithelial cells in culture. In Vitro Cell. Dev. Biol. 22: 738-748, 1986. PubMed: 3782011

Ehmann UK, et al. Cultured proliferating rat mammary epithelial cells. In Vitro Cell. Dev. Biol. 27A: 749-754, 1991. PubMed: 1717432

Ehmann UK, et al. Gap-junctional communication between feeder cells and recipient normal epithelial cells correlates with growth stimulation. In Vitro Cell. Dev. Biol. Anim. 37: 100-110, 2001. PubMed: 11332735

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ehmann UK, et al. To grow mouse mammary epithelial cells in culture. J. Cell Biol. 98: 1026-1032, 1984. PubMed: 6699079

Dulbecco R, et al. Differentiation of a rat mammary cell line in vitro. Proc. Natl. Acad. Sci. USA 76: 1256-1260, 1979. PubMed: 375233

Ehmann UK, et al. Long-term proliferation of mouse thymic epithelial cells in culture. In Vitro Cell. Dev. Biol. 22: 738-748, 1986. PubMed: 3782011

Ehmann UK, et al. Cultured proliferating rat mammary epithelial cells. In Vitro Cell. Dev. Biol. 27A: 749-754, 1991. PubMed: 1717432

Ehmann UK, et al. Gap-junctional communication between feeder cells and recipient normal epithelial cells correlates with growth stimulation. In Vitro Cell. Dev. Biol. Anim. 37: 100-110, 2001. PubMed: 11332735

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm