LA7 (ATCC® CRL-2283™)

Organism: Rattus norvegicus, rat  /  Cell Type: Epithelial

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Rattus norvegicus, rat
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Strain Sprague-Dawley
Applications
LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA.
Cells irradiated with 6000 rads may be used as feeder layers.
Derivation
LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA.
It is a clonal derivative of the Rama 25 line obtained from a rat treated with dimethylbenz(a)anthracene.
Clinical Data
LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA.
female
Comments
LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA.
It is a clonal derivative of the Rama 25 line obtained from a rat treated with dimethylbenz(a)anthracene.
Cells irradiated with 6000 rads may be used as feeder layers.
Such feeder layers will stimulate the proliferation of normal primary mouse and rat mammary cells and normal primary mouse thymic epithelial cells.
Complete Growth Medium Dulbecco's modified Eagle's medium with reduced sodium bicarbonate (1.5 to 2.0 g/L), 4.5 g/L glucose, 0.005 mg/ml insulin, 50 nM hydrocortisone and 20 mM HEPES, 95%; fetal bovine serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor UK Ehmann
Year of Origin 1979
References

Ehmann UK, et al. To grow mouse mammary epithelial cells in culture. J. Cell Biol. 98: 1026-1032, 1984. PubMed: 6699079

Dulbecco R, et al. Differentiation of a rat mammary cell line in vitro. Proc. Natl. Acad. Sci. USA 76: 1256-1260, 1979. PubMed: 375233

Ehmann UK, et al. Long-term proliferation of mouse thymic epithelial cells in culture. In Vitro Cell. Dev. Biol. 22: 738-748, 1986. PubMed: 3782011

Ehmann UK, et al. Cultured proliferating rat mammary epithelial cells. In Vitro Cell. Dev. Biol. 27A: 749-754, 1991. PubMed: 1717432

Ehmann UK, et al. Gap-junctional communication between feeder cells and recipient normal epithelial cells correlates with growth stimulation. In Vitro Cell. Dev. Biol. Anim. 37: 100-110, 2001. PubMed: 11332735

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Ehmann UK, et al. To grow mouse mammary epithelial cells in culture. J. Cell Biol. 98: 1026-1032, 1984. PubMed: 6699079

Dulbecco R, et al. Differentiation of a rat mammary cell line in vitro. Proc. Natl. Acad. Sci. USA 76: 1256-1260, 1979. PubMed: 375233

Ehmann UK, et al. Long-term proliferation of mouse thymic epithelial cells in culture. In Vitro Cell. Dev. Biol. 22: 738-748, 1986. PubMed: 3782011

Ehmann UK, et al. Cultured proliferating rat mammary epithelial cells. In Vitro Cell. Dev. Biol. 27A: 749-754, 1991. PubMed: 1717432

Ehmann UK, et al. Gap-junctional communication between feeder cells and recipient normal epithelial cells correlates with growth stimulation. In Vitro Cell. Dev. Biol. Anim. 37: 100-110, 2001. PubMed: 11332735