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VA-ES-BJ (ATCC^® CRL-2138^™)

Organism: Homo sapiens, human / Cell Type: Epithelial

VA-ES-BJ ATCC^® CRL-2138^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Cell Type	Epithelial
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1
Disease	epithelioid carcinoma
Age	41 years
Gender	male
Ethnicity	Caucasian
Applications	VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an adult male with primary vertebral epithelioid carcinoma. The cells are sensitive to hemin. The cell are positive for immunohistochemical staining for the following: AE-1 and AE-3 cytokeratins, fas, epithelial membrane antigen (EMA), vimentin and p53.

Karyotype	near triploid karyotype with structural as well as numerical abnormalities; modal number = 65. Rearrangements of both chromosomes 1 and 9 with unknown materials were present., Five copies of chromosome 7 were seen in almost every cell, and there was no intact Y chromosome present. Cell to cell variation was due to numerical differences only, structural abnormalities were very consistent.
Derivation	VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an adult male with primary vertebral epithelioid carcinoma.
Clinical Data	VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an adult male with primary vertebral epithelioid carcinoma. male Caucasian 41 years
Oncogene	p53 +
Genes Expressed	granulocyte macrophage colony stimulating factor (GM-CSF),p53 +
Tumorigenic	Yes
Comments	VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an adult male with primary vertebral epithelioid carcinoma. The cells are sensitive to hemin. The cell are positive for immunohistochemical staining for the following: AE-1 and AE-3 cytokeratins, fas, epithelial membrane antigen (EMA), vimentin and p53.

Complete Growth Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.1 mM non-essential amino acids; fetal bovine serum, 10%
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: Twice per week Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation	Culture medium, 95%; DMSO, 5%

Name of Depositor	C Helson
Year of Origin	1991
References	. . Int. J. Oncol. 7: 51-56, 1995.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
References	. . Int. J. Oncol. 7: 51-56, 1995.