HPAF-II (ATCC® CRL-1997)

Organism: Homo sapiens, human  /  Tissue: pancreas  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Tissue pancreas
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 44 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
HPAF-II is a human pancreatic adenocarcinoma cell line derived from peritoneal ascitic fluid of a 44 year old Caucasian male with primary pancreatic adenocarcinoma and metastases to the liver, diaphragm and lymph nodes.­
Clinical Data
Caucasian
44 years
male
Antigen Expression
Blood Type A; Rh-; HLA A1, A10 (W34), B8, W22, Cw3
Genes Expressed
mucin
Tumorigenic Yes
Effects
Yes, the cells form well differentiated adenocarcinomas in athymic mice which resemble the original tumor
Comments

HPAF II is a spontaneous variant with unlimited replicative capability which proliferated from a static culture of HPAF-I cells. The cells express Muc 1 and Muc 4 mucin genes, and secrete high levels of Muc 1 mucin. They are pleomorphic and probably undergo some spontaneous differentiation in culture.

 

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 11,13
D5S818: 11,13
D7S820: 10,13
F13A01: 5,17
F13B: 8,10
FESFPS: 11,12
LPL: 10
THO1: 9
TPOX: 8
vWA: 17
Name of Depositor RS Metzgar
References

Monoclonal antibodies in cancer. Clifton, NJ: Humana Press; 1985.

Monoclonal antibodies and cancer therapy. New York: Liss; 1985.

Immunity to cancer II. New York: Liss; 1989.

Metzgar RS, et al. Antigens of human pancreatic adenocarcinoma cells defined by murine monoclonal antibodies. Cancer Res. 42: 601-608, 1982. PubMed: 7034925

Kim YW, et al. Characterization of clones of a human pancreatic adenocarcinoma cell line representing different stages of differentiation. Pancreas 4: 353-362, 1989. PubMed: 2734279

Mullins TD, et al. Ultrastructural differentiation of sodium butyrate-treated human pancreatic adenocarcinoma cell lines. Pancreas 6: 578-587, 1991. PubMed: 1946315

Worlock AJ, et al. Radiolocalization of human pancreatic tumors in athymic mice by monoclonal antibody DU-PAN 1. Cancer Res. 50: 7246-7251, 1990. PubMed: 2224857

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Monoclonal antibodies in cancer. Clifton, NJ: Humana Press; 1985.

Monoclonal antibodies and cancer therapy. New York: Liss; 1985.

Immunity to cancer II. New York: Liss; 1989.

Metzgar RS, et al. Antigens of human pancreatic adenocarcinoma cells defined by murine monoclonal antibodies. Cancer Res. 42: 601-608, 1982. PubMed: 7034925

Kim YW, et al. Characterization of clones of a human pancreatic adenocarcinoma cell line representing different stages of differentiation. Pancreas 4: 353-362, 1989. PubMed: 2734279

Mullins TD, et al. Ultrastructural differentiation of sodium butyrate-treated human pancreatic adenocarcinoma cell lines. Pancreas 6: 578-587, 1991. PubMed: 1946315

Worlock AJ, et al. Radiolocalization of human pancreatic tumors in athymic mice by monoclonal antibody DU-PAN 1. Cancer Res. 50: 7246-7251, 1990. PubMed: 2224857