UC7-13D5 (ATCC® CRL-1989)

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the murine T cell receptor gamma/delta TCR.
The antibody is specific for all murine TCR gamma/delta heterodimers.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR)
Comments
Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the murine T cell receptor gamma/delta TCR.
The antibody is specific for all murine TCR gamma/delta heterodimers.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
90% complete growth medium, 10% DMSO.
Isotype IgG
Name of Depositor JA Bluestone
References

Bluestone JA, et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol. Rev. 120: 5-33, 1991. PubMed: 1650760

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Bluestone JA, et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol. Rev. 120: 5-33, 1991. PubMed: 1650760