IgG-5D7 [anti LRP 515] (ATCC® CRL-1938)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts specifically with the 515 kDa subunit of rabbit LRP.
Derivation
Animals were immunized with purified rabbit LRP.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the 515000 dalton (515 kDa) subunit of rabbit lipoprotein receptor related protein (LRP)
Cellular Products
immunoglobulin; monoclonal antibody; against the 515000 dalton (515 kDa) subunit of rabbit lipoprotein receptor related protein (LRP)
Comments
Animals were immunized with purified rabbit LRP.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts specifically with the 515 kDa subunit of rabbit LRP.
It does not react with the 85 kDa subunit of rabbit LRP, nor does it cross-react with the 515 kDa subunit of rat LRP.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 1 to 2 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor YK Ho, JL Goldstein
References

Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029