PDL-1 (ATCC® CRL-1882)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the cell surface of periodontal ligament cells of bovine and baboon origin.
The antibody also reacts with cells of mesenchymal origin from a number of organs.
Derivation
Spleen cells were fused with FOX-NY myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a cell surface antigen on bovine periodontal ligament cells
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with cultured bovine periodontal ligament cells.
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with the cell surface of periodontal ligament cells of bovine and baboon origin.
The antibody also reacts with cells of mesenchymal origin from a number of organs.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor RJ Klebe
References

DuBois WT, et al. Monoclonal antibodies to periodontal ligament cells. J. Periodontol. 62: 190-196, 1991. PubMed: 2027070

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

DuBois WT, et al. Monoclonal antibodies to periodontal ligament cells. J. Periodontol. 62: 190-196, 1991. PubMed: 2027070