1B8-F11 (ATCC® CRL-1852)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody is specific for the gD glycoprotein of BHV-1 and neutralizes the virus in the absence of complement.
It is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine herpesvirus I (BHV-1); also known as infectious bovine rhinotracheitis virus (IBRV)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with BHV-1.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the gD glycoprotein of BHV-1 and neutralizes the virus in the absence of complement.
It is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor CA Whetstone
References

. . Conf. Res. Workers Anim. Dis. : 54, 1987.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Conf. Res. Workers Anim. Dis. : 54, 1987.