Wgd5 (ATCC® CRL-1817)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  /  Disease: Leukemia

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Disease Leukemia
Applications
transfection host
Helper cell line for packaging retroviral vectors such as those released from lambdaZd31, lambdaZd32, and lambdaZd35 (ATCC 37563-5, respectively).
Derivation
The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus).
Genes Expressed
retrovirus vector (Moloney murine leukemia virus
Comments
The Wgd5 cell line contains a mutant of the Moloney murine leukemia virus (MoMuLV) that is useful for packaging recombinant retroviral DNA into infectious virus particles.
The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus).
Wgd5 was selected as a gpt+ clone after cotransformation of WOP with pSV2gpt and a packaging mutant of MoMuLV.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 0.25 mg/ml xanthine, 0.015 mg/ml hypoxanthine, 0.01 mg/ml thymidine, 0.002 mg/ml aminopterin and 0.015 mg/ml mycophenolic acid, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, rinse and remove trypsin.
Allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes), add fresh medium, aspirate and dispense into new flasks.
Subculture before the cells become confluent.
If allowed to become confluent, the cells will slough off and die.
Name of Depositor AJ Murphy
References

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791