Psi2 12S6 (ATCC® CRL-1808)

Organism: Mus musculus, mouse  /  Cell Type: Epithelial  / 

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Organism Mus musculus, mouse
Cell Type Epithelial
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Strain NIH/Swiss
Applications
This construct has been used to immortalize primary epithelial cells of various rat tissues including kidney, liver, heart, pancreas and thyroid.
The line was produced by inserting the E1A 12S cDNA sequence into the retrovirus vector DOL.
The retroviruses produced during the first 24 hours post-transfection were harvested and used to infect fresh Psi2 cells to generate the Psi2 12S6 line.
The retrovirus produced by this line can be used to to immortalize primary epithelial cells of various rat tissues including kidney, liver, heart, pancreas and thyroid.
Tested and found negative for ectromelia virus (mousepox).
Shipping Information Distributed: Frozen
Derivation
The line was produced by inserting the E1A 12S cDNA sequence into the retrovirus vector DOL.
The retroviruses produced during the first 24 hours post-transfection were harvested and used to infect fresh Psi2 cells to generate the Psi2 12S6 line.
The retrovirus produced by this line can be used to to immortalize primary epithelial cells of various rat tissues including kidney, liver, heart, pancreas and thyroid.
HeLa Markers N
Genes Expressed
a retrovirus vector containing the adenovirus 12S E1A and the neomycin phosphotransferase genes,early-region 1A, 12S [E1A]
Cellular Products
a retrovirus vector containing the adenovirus 12S E1A and the neomycin phosphotransferase genes
Comments
The 13S E1A mRNA encoded by rv13S (ATCC CRL-1809) is 46 amino acids longer than the 12S E1A mRNA encoded by rv12S (ATCC CRL-1808).
This construct has been used to immortalize primary epithelial cells of various rat tissues including kidney, liver, heart, pancreas and thyroid.
The line was produced by inserting the E1A 12S cDNA sequence into the retrovirus vector DOL.
The resulting vector DNA (rv12S) was transfected into Psi2 cells by calcium phosphate precipitation.
The retroviruses produced during the first 24 hours post-transfection were harvested and used to infect fresh Psi2 cells to generate the Psi2 12S6 line.
The retrovirus produced by this line can be used to to immortalize primary epithelial cells of various rat tissues including kidney, liver, heart, pancreas and thyroid.
Tested and found negative for ectromelia virus (mousepox).
The insert contains sequences from adenovirus type 2-adenovirus type 5 hybrid cDNAs.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:40 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin solution for several minutes, remove trypsin and allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor RD Cone
References

Cone RD, et al. A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissue. Mol. Cell. Biol. 8: 1036-1044, 1988. PubMed: 2966895

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Cone RD, et al. A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissue. Mol. Cell. Biol. 8: 1036-1044, 1988. PubMed: 2966895