3C9-D11-H11 (ATCC® CRL-1745)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Mice were immunized with purified PPV.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV)
Cellular Products
immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Mice were immunized with purified PPV.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody has both virus neutralization and hemagglutinating activities.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor RA Van Deusen
References

. 8th Proc. Intl. Pig. Vet. Soc.. Gent, Belgium: ; 1984.

Katz JB, van Deusen RA. Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system. J. Virol. Methods 12: 193-198, 1985. PubMed: 3009510

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. 8th Proc. Intl. Pig. Vet. Soc.. Gent, Belgium: ; 1984.

Katz JB, van Deusen RA. Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system. J. Virol. Methods 12: 193-198, 1985. PubMed: 3009510