MC116 (ATCC® CRL-1649)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: ascites  /  Disease: undifferentiated lymphoma

Organism Homo sapiens, human
Tissue ascites
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease undifferentiated lymphoma
Gender male
Karyotype 45,XO/46,XY, dup1(q21-32), t(8;14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23)
Clinical Data
male
Antigen Expression
HLA A1, A3, B8, Bw35
Genes Expressed
immunoglobulin (surface and secreted)
Cellular Products
immunoglobulin (surface and secreted)
Comments
The cells are EBNA negative, negative for Fc receptors and negative for complement receptors.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 105 viable cells/mL and maintain at or below 3 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Isotype IgM; lambda light chain
Population Doubling Time 26 to 28 hrs
Name of Depositor IT Magrath
References

Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722

Cross References

Nucleotide (GenBank) : U07991 Human lymphoma immunoglobulin lambda light chain mRNA, partial cds.

Nucleotide (GenBank) : U07988 Human lymphoma immunoglobulin IgM heavy chain Fd fragment mRNA, partial cds.

Basic Documentation
References

Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722