H-4-II-E (ATCC® CRL-1548)

Organism: Rattus norvegicus, rat  /  Tissue: liver  /  Disease: hepatoma

Organism Rattus norvegicus, rat
Tissue liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatoma
Applications
The line is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts.
Genes Expressed
­
Cellular Products
aryl hydrocarbon hydroxylase is inducible
Comments
The line has been cured of Mycoplasma arginini contamination.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor JA Bradlaw
Product Origin http://prod-cms.atcc.org/sitecore/shell/Applications/Content%20editor.aspx#
References

Benedict WF, et al. Aryl hydrocarbon hydroxylase induction in mammalian cell culture--IV. Stimulation of the enzyme activity in established cell lines derived from rat or mouse hepatoma and from normal rat liver. Biochem. Pharmacol. 22: 2766-2769, 1973. PubMed: 4357894

. . In Vitro 16: 230, 1980.

Sassier P, Bergeron M. Specific inhibition of cell proliferation in the mouse intestine by an aqueous extract of rabbit small intestine. Cell Tissue Kinet. 10: 223-243, 1977. PubMed: 872181

Bradlaw JA, Casterline JL Jr.. Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. J. Assoc. Off. Anal. Chem. 62: 904-916, 1979. PubMed: 115836

Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233

Cross References

Nucleotide (GenBank) : U49128 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3b (delta part IVS4), partial cds.

Nucleotide (GenBank) : U49126 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3 (delta IVS3 delta IVS4), partial cds.

Nucleotide (GenBank) : U49127 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3 (delta IVS3 delta part IVS4), partial cds.

Basic Documentation
References

Benedict WF, et al. Aryl hydrocarbon hydroxylase induction in mammalian cell culture--IV. Stimulation of the enzyme activity in established cell lines derived from rat or mouse hepatoma and from normal rat liver. Biochem. Pharmacol. 22: 2766-2769, 1973. PubMed: 4357894

. . In Vitro 16: 230, 1980.

Sassier P, Bergeron M. Specific inhibition of cell proliferation in the mouse intestine by an aqueous extract of rabbit small intestine. Cell Tissue Kinet. 10: 223-243, 1977. PubMed: 872181

Bradlaw JA, Casterline JL Jr.. Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. J. Assoc. Off. Anal. Chem. 62: 904-916, 1979. PubMed: 115836

Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233