CCD-18Co (ATCC® CRL-1459)

Organism: Homo sapiens, human  /  Tissue: colon  /  Disease: normal

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Organism Homo sapiens, human
Tissue
colon
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 2.5 months
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor temperature
Clinical Data
2.5 months
Black
female
Comments
The line begins to senesce at about PDL = 42.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Growth Conditions: Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
STR Profile
Amelogenin: X
CSF1PO: 8
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 8
THO1: 6,7
TPOX: 8,11
vWA: 15,17
Population Doubling Time The line begins to senesce at about PDL = 42.
Name of Depositor MI Cour
References

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

Hinterleitner TA, et al. Il-1 stimulates intestinal myofibroblast COX gene expression and augments activation of CI- secretion in T84 cells. Am. J. Physiol. 271: C1262-C1268, 1996. PubMed: 8897833

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

Hinterleitner TA, et al. Il-1 stimulates intestinal myofibroblast COX gene expression and augments activation of CI- secretion in T84 cells. Am. J. Physiol. 271: C1262-C1268, 1996. PubMed: 8897833