DDT1-MF-2 (ATCC® CRL-12051)

Organism: Mesocricetus auratus, hamster, Syrian golden  /  Disease: leiomyosarcoma

Organism Mesocricetus auratus, hamster, Syrian golden
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease leiomyosarcoma
Gender male
Clinical Data
male
Receptor Expression
androgen receptor, positive; glucocorticoid; alpha 1 adrenergic; beta 2 adrenergic
Comments
Growth of DDT1-MF-2 cells is stimulated by androgens and inhibited by glucocorticoids.
Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene.
Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase.
This a deposit of DDT1-MF-2 (see ATCC CRL-1701) for patent purposes.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week
To dislodge adherent cells, remove all but 5 to 10 ml of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Name of Depositor Merck & Co., Inc.
References

Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513

Basic Documentation
References

Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513