WISH (ATCC® CCL-25™)

Organism: Homo sapiens, human  /  Tissue: HeLa contaminant  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue HeLa contaminant
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2
Derivation
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
HeLa Markers Y
Genes Expressed
The cells are positive for keratin by immunoperoxidase staining.
The cells and tumors formed from the cells are positive for Dopa decarboxylase and are negative for the TAG-72 antigen.
Comments Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor L Hayflick
References

Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490

. . Am. J. Dis. Child. 103: 460, 1962.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Additional Information
References

Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490

. . Am. J. Dis. Child. 103: 460, 1962.