R9ab (ATCC® CCL-193)

Organism: Oryctolagus cuniculus, rabbit  /  Cell Type: fibroblast  / 

Permits and Restrictions

View Permits

Organism Oryctolagus cuniculus, rabbit
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age fetus
Gender female
Applications
Subsequent cell outgrowths were trypsinized and the resulting cell suspension was used to initiate the line.
R9ab was derived from explants of biopsy material.
Karyotype normal; diploid; stable
Derivation
R9ab was derived from explants of biopsy material. Subsequent cell outgrowths were trypsinized and the resulting cell suspension was used to initiate the line.
Clinical Data
The donor indicated that the life expectancy of this line is at least 60 passages.
female
Comments
R9ab was derived from explants of biopsy material. Subsequent cell outgrowths were trypsinized and the resulting cell suspension was used to initiate the line.
The donor indicated that the life expectancy of this line is at least 60 passages.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor EM Earley
Passage History
The donor indicated that the life expectancy of this line is at least 60 passages.
References

Castagliuolo I, et al. Saccharomyces boulardii protease inhibits Clostridium difficile toxin A effects in the rat ileum. Infect. Immun. 64: 5225-5232, 1996. PubMed: 8945570

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Castagliuolo I, et al. Saccharomyces boulardii protease inhibits Clostridium difficile toxin A effects in the rat ileum. Infect. Immun. 64: 5225-5232, 1996. PubMed: 8945570