RPMI 6666 (ATCC® CCL-113)

Organism: Homo sapiens, human  /  Tissue: peripheral blood  /  Disease: Hodgkin's disease; Hodgkin's lymphoma

Organism Homo sapiens, human
Tissue peripheral blood
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 [Cell contain Herpesvirus]
Disease Hodgkin's disease; Hodgkin's lymphoma
Age 29 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data 29 years
Caucasian
male
Antigen Expression
HLA A2, A3, B7, B18
Genes Expressed
Immunoglobulin
Cellular Products
Immunoglobulin
Comments
The cells are EBNA positive.
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL.
Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days.
Cryopreservation Freeze medium: complete growth medium, 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Name of Depositor GE Moore
References

Moore GE, et al. Leukocyte cultures of patients with leukemia and lymphomas. N.Y. State J. Med. 66: 2757-2764, 1966. PubMed: 5232150

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Basic Documentation
References

Moore GE, et al. Leukocyte cultures of patients with leukemia and lymphomas. N.Y. State J. Med. 66: 2757-2764, 1966. PubMed: 5232150

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.