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Cell Biology

  • CoolCell® LX Alcohol-Free Cryopreservation Container (ATCC® ACS-6000)

    ATCC® Number: ACS-6000
    Applications:

    The CoolCell® LX container, in combination with a -80°C freezer or dry ice locker, will provide the freezing rate of -1°C per minute that is ideal for cryo-preservation of most cultured cell lines.  

    For-Profit:   $149.00 Non-Profit:   $149.00

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  • RPMI-1640 Medium (ATCC® 30-2001)

    ATCC® Number: 30-2001
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Trypsin-EDTA Solution, 1X (ATCC® 30-2101)

    ATCC® Number: 30-2101
    Quantity: 100 mL
    Storage: Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications:

    0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.

    For-Profit:   $13.00 Non-Profit:   $13.00

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    Product Format: frozen 100 mL
    Comments: NOTE:  Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner.  For optimum results, observe the cells during the dissociation process to avoid over-trypsinization.  For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution.  For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service.  ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines.
  • ITS Solution (Insulin, Transferrin, Selenious Acid) (ATCC® 30-2606)

    ATCC® Number: 30-2606
    Quantity: 10 mL
    Storage: Store medium at 2°C to 8°C. Do not freeze.
    Applications:

    Insulin, Transferrin and Selenious Acid (ITS) have been demonstrated to improve performance in serum-free media. Insulin promotes the uptake of glucose and amino acids. Transferrin is an iron-transport protein, and with selenious acid, supplies essential trace elements normally provided by serum. ITS Solution is free of animal origin.

    For-Profit:   $43.00 Non-Profit:   $43.00

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    Product Format: 10 mL
    Comments:

    Supplied at a 100x concentration. For use, aseptically add 5 mL of ITS solution per 500 mL of medium.

  • Hybri-Care Medium (ATCC® 46-X)

    ATCC® Number: 46-X
    Quantity: 1 Package (prepares 1L of medium)
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines.
    A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 1 Package (prepares 1L of medium)
    Comments:
    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. Ingredients are essentially those of a modified DMEM and NCTC 135 plus insulin. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    When used with 10% fetal bovine serum, Hybri-Care repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection.

    Ozato, K., et al. J. Immunology 124: 533-540, 1980.
  • Fetal Bovine Serum, ES Cell Qualified (ATCC® SCRR-30-2020)

    ATCC® Number: SCRR-30-2020
    Quantity: 500 mL
    Storage: Store fetal bovine serum at -70°C in its yellow plastic storage bag. The yellow bag minimizes the effect of light on the serum. If storage at -70°C is not possible, then store at -20°C. Do not store fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications:

    This serum is tested for ability to support the growth and maintenance of embryonic stem cells in an undifferentiated state. Undifferentiated embryonic stem cells are determined by colony morphology and expression of three pluripotent markers. The results are compared to a control lot of serum. The serum is triple filtered through 0.1µM filters and each lot of fetal bovine serum is tested for sterility. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States.


    For-Profit:   $424.00 Non-Profit:   $424.00

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    Product Format: frozen 500 mL
    Comments: The results were compared with cells grown with a control lot of serum.
  • Fetal Bovine Serum (ATCC® 30-2020)

    ATCC® Number: 30-2020
    Quantity: 500 mL
    Storage: Store fetal bovine serum at -20°C or colder. Do not store frozen fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications: Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

    For-Profit:   $324.00 Non-Profit:   $324.00

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  • McCoy's 5A Medium (ATCC® 30-2007)

    ATCC® Number: 30-2007
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    McCoy's 5A Medium is modified to contain 1.5 mM L-glutamine and 2200 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 2.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.


  • DMEM: F-12 Medium (ATCC® 30-2006)

    ATCC® Number: 30-2006
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • MEM Nonessential Amino Acid Solution, 100x Solution (ATCC® 30-2116)

    ATCC® Number: 30-2116
    Quantity: 100 mL
    Storage: Store MEM Nonessential Amino Acid Solution at 2°C to 8°C
    Applications:

    Added as a supplement to minimal basal media. The nonessential amino acids in this solution are 100 times the concentration found in MEM-alpha Medium.

    For-Profit:   $14.00 Non-Profit:   $14.00

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    Formulation: Formulation
    Product Format: 100 mL
    Comments:
    Added as a supplement to minimal basal media. Contains the nonessential amino acids found in MEM-alpha medium at 100x concentration.