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Cell Biology

  • Dulbecco's Phosphate Buffered Saline (D-PBS), 1X (ATCC® 30-2200)

    ATCC® Number: 30-2200
    Quantity: 500 mL
    Storage: Store Dulbecco's PBS at ambient temperatures, between 15°C and 30°C.
    Applications:

    To be used for washing cells,  as a diluent, or as an inorganic base for standard media preparation.

    For-Profit:   $15.00 Non-Profit:   $15.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    Formulation contains no calcium or magnesium.

  • Calf Bovine Serum (ATCC® 30-2031)

    ATCC® Number: 30-2031
    Quantity: 100 mL
    Storage: Store calf bovine serum at -20°C or colder. Do not store frozen calf bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum in aliquots. Calf bovine serum is supplied in PETG plastic bottles.
    Applications: Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $21.00 Non-Profit:   $21.00

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  • AlphaMEM with Nucleosides without L-Glutamine. (ATCC® 30-2604)

    ATCC® Number: 30-2604
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    AlphaMEM (Alpha Minimum Essential Medium) is an enriched modification of MEM that is widely used in mammalian cell culture. This formulation is made in Earle’s salts and contains ribonucleosides and deoxyribonucleosides.

    For-Profit:   $31.00 Non-Profit:   $31.00

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    Product Format: 500 mL
    Comments: Supplement with 2 mM L-glutamine prior to use.
  • Trypsin-EDTA Solution, 1X (ATCC® 30-2101)

    ATCC® Number: 30-2101
    Quantity: 100 mL
    Storage: Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications:

    0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.

    For-Profit:   $13.00 Non-Profit:   $13.00

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    Product Format: frozen 100 mL
    Comments: NOTE:  Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner.  For optimum results, observe the cells during the dissociation process to avoid over-trypsinization.  For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution.  For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service. ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product contains phenol red.
  • Fetal Bovine Serum (ATCC® 30-2020)

    ATCC® Number: 30-2020
    Quantity: 500 mL
    Storage: Store fetal bovine serum at -20°C or colder. Do not store frozen fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications: Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

    For-Profit:   $324.00 Non-Profit:   $324.00

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  • Eagle's Minimum Essential Medium (EMEM) (ATCC® 30-2003)

    ATCC® Number: 30-2003
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: Modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $19.00 Non-Profit:   $19.00

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    Product Format: 500 mL
    Comments: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • RPMI Medium without Phenol Red without L-Glutamine (ATCC® 30-2602)

    ATCC® Number: 30-2602
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    RPMI 1640 is a modification of McCoy’s 5A medium that contains a higher concentration of inositol. The medium is commonly used with mammalian myeloma and hybridoma cells. This formulation does not contain phenol red or L-glutamine.

    For-Profit:   $21.00 Non-Profit:   $21.00

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    Product Format: 500 mL
    Comments:

    Supplement with 2 mM L-glutamine prior to use. Modified to contain 10 mM HEPES, 1 mM sodium pyruvate, 4.5 g/L D-glucose, and 1.5 g/L sodium bicarbonate. Note: This reduced level of sodium bicarbonate is intended for use in a 5% COin air environment. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

  • Dulbecco's Modified Eagle's Medium (DMEM) without Phenol Red (ATCC® 30-2601)

    ATCC® Number: 30-2601
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) is a basal medium widely used in mammalian cell culture. The medium is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins. This formulation does not contain phenol red or L-glutamine.

    For-Profit:   $21.00 Non-Profit:   $21.00

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    Product Format: 500 mL
    Comments: Supplement with 4 mM L-glutamine prior to use.
  • CoolCell® LX Alcohol-Free Cryopreservation Container (ATCC® ACS-6000)

    ATCC® Number: ACS-6000
    Applications:

    The CoolCell® LX container, in combination with a -80°C freezer or dry ice locker, will provide the freezing rate of -1°C per minute that is ideal for cryo-preservation of most cultured cell lines.  

    For-Profit:   $149.00 Non-Profit:   $149.00

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  • ITS Solution (Insulin, Transferrin, Selenious Acid) (ATCC® 30-2606)

    ATCC® Number: 30-2606
    Quantity: 10 mL
    Storage: Store medium at 2°C to 8°C. Do not freeze.
    Applications:

    Insulin, Transferrin and Selenious Acid (ITS) have been demonstrated to improve performance in serum-free media. Insulin promotes the uptake of glucose and amino acids. Transferrin is an iron-transport protein, and with selenious acid, supplies essential trace elements normally provided by serum. ITS Solution is free of animal origin.

    For-Profit:   $43.00 Non-Profit:   $43.00

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    Product Format: 10 mL
    Comments:

    Supplied at a 100x concentration. For use, aseptically add 5 mL of ITS solution per 500 mL of medium.