DNA from HCMV strain AD-169 [ATCC VR-538] (ATCC® VR-538D)

Organism: Human herpesvirus 5, Human cytomegalovirus  / 

Product Format frozen Total DNA from infected cells, 100 µL per vial
Storage Conditions -70°C or colder
Applications
It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Comments
This product is a preparation of viral nucleic acids, including virus genomic DNA, in total DNA from MRC-5 cells (ATCC CCL-171) infected with Human herpesvirus 5 (HCMV) strain AD-169 (VR-538). The product is supplied at 100 ul per vial, shipped frozen. It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Strain AD-169 has been completely sequenced. Genbank: X17403
Biosafety Level 1
Quality Control Specifications
  1. Store at -20°C or colder. -80°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).
  2. Concentration is verified as > 1 µg/mL DNA by Picogreen® assay.
  3. Purity is confirmed by OD260/OD280 ratio (> 1.7) of bulk DNA prior to dilution for product filling.
  4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis 20 uL of product on a 0.8% agarose gel, visualized by ethidium bromide staining.
  5. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.
  6. Identity is confirmed by sequencing of ~300 base pairs of the PCR amplicon.
  7. Absence of infectious virus is verified by incubation of 10% of each lot with susceptible host cells under appropriate conditions for a period of 7-14 days.