DNA from Human adenovirus 1 strain Adenoid 71 [ATCC VR-1] (ATCC® VR-1D™)

Organism: Human adenovirus 1  / 

Product Format frozen

PRODUCT INFORMATION:

Total DNA from infected cells, >1 µg/mL in 1X TE buffer

100 µL per vial, shipped frozen

OD260/OD280: > 1.7

Storage Conditions -70°C or colder
Applications
It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Respiratory research
Comments
This product is a preparation of viral nucleic acids, including virus genomic DNA, in total DNA isolated from A549 cells (ATCC CCL-185) infected with Human adenovirus 1 (HAdV-1) strain Adenoid 71 (VR-1). It is supplied at 100 ul per vial, shipped frozen. It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Focus Area (for hierarchy)
Infectious diseases
Biosafety Level 1
Quality Control Specifications
1. Store at -20°C or colder. -80°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).

2. Concentration is verified as > 1 µg/mL DNA by Picogreen® assay.

3. Purity is confirmed by OD260/OD280 ratio (> 1.7) of bulk DNA prior to dilution for product filling.

4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µl of product on a 0.8% agarose gel, visualized by ethidium bromide staining.

5. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.

6. Identity is confirmed by sequencing of ~300 base pairs of the PCR amplicon.

7. Absence of infectious virus is verified by incubation of 10% of each lot with susceptible host cells under appropriate conditions for a period of 7-14 days.