RNA from Human respiratory syncytial virus strain A2 (ATCC® VR-1540D)

Organism: Human respiratory syncytial virus  / 

Permits and Restrictions

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Product Format frozen
Total genomic RNA from infected cells, 100 µL per vial, shipped frozen
Storage Conditions -70°C or colder
The vapor-phase of liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).
Applications
It is suitable for use in reverse transcriptase-polymerase chain reaction (RT-PCR) for viral gene products and other molecular virology applications.
Comments
This product is a preparation of viral nucleic acids, including virus genomic RNA, in total RNA isolated from HEp-2 cells (ATCC CCL-23) infected with Human respiratory syncytial virus (HRSV) strain A2 (ATCC VR-1540). It is supplied at 100 µL per vial, shipped frozen. It is suitable for use in reverse transcriptase-polymerase chain reaction (RT-PCR) for viral gene products and other molecular virology applications.
Biosafety Level 1
Quality Control Specifications
  1. Concentration is verified as ≥ 1µg/mL by Ribogreen® assay.
  2. Integrity is inferred from observation of intact RT-PCR product on a 1.2% agarose gel, visualized by ethidium bromide staining.
  3. Functional activity is demonstrated by RT-PCR amplification of ≥ 500 bp amplicon dilutable ≥ 10 fold.
  4. Identity is confirmed by sequence analysis of the RT-PCR amplicon.
  5. Virus inactivation is verified by the incubation of 10% of total yield on susceptible host cells followed by evaluation for cytopathic effect.
  6. Purity is confirmed by OD260/OD280 ratio (≥ 1.8) of bulk RNA prior to dilution for product filling.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation