Quantitative Synthetic Hepatitis B virus DNA (ATCC® VR-3232SD)

Product Format: frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica DNAstable

Permits and Restrictions

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Agent Quantitative Synthetic Hepatitis B virus DNA
Applications ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day to day test variation, and lot to lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica DNAstable
Storage Conditions -20°C or colder
Intended Use The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

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Comments Manufactured under ISO 13485 guidance

Preparation includes fragments from the highly conserved precore, core, P, S, and X regions.
Special Collection DNA

Sun S, et al. Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection. Virol. J. 8: 227, 2011. PubMed: 21569595

Welzel TM, et al. Real-time PCR assay for detection and quantification of hepatitis B virus genotypes A to G. J. Clin. Microbiol. 44(9): 3325-3333, 2006. PubMed: 16954268

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Test primers and probe for synthetic Hepatitis B DNA VR-3232SD

    ATCC used the following primers and probe to obtain a positive PCR product from this DNA:

    Date Updated: 8/25/2015
  2. Subtype of synthetic hepatitis B VR-3232SD

    There is no one subtype for this synthetic DNA but instead it was made by doing  multiple sequence alignments of the available genomes from NCBI and is synthetic mixture.

    Date Updated: 10/21/2015