Porcine respiratory coronavirus ATCC ® VR-2384™

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Agent	Porcine respiratory coronavirus
Strain	AR310
Applications	This strain provides a biological pure culture of a novel pathogenic porcine respiratory coronavirus (PRCV) and a vaccine derived therefrom effective against PRCV infection and transmissible gastroenteritis virus (TGEV) infection. The virus produces moderate pneumonia in pigs, there is no indication of human health hazard.
Biosafety Level	2
Isolation	Culture of a novel TGEV-related Porcine Respiratory Coronavirus
Product Format	frozen
Storage Conditions	-70°C or colder

Comments	This strain provides a biological pure culture of a novel pathogenic porcine respiratory coronavirus (PRCV) and a vaccine derived therefrom effective against PRCV infection and transmissible gastroenteritis virus (TGEV) infection. The virus produces moderate pneumonia in pigs, there is no indication of human health hazard.
Effect on Host	Yes, in vitro effects: Cytopathic effects (fused and rounded cells) 18 to 24 hours post-inoculation CPE, fused and rounded cells 18-24 hours post-inculation

Recommended Host	swine testicular (ST) cells (ATCC CRL-1746) Recommended Host: Swine testicular cells (ATCC^® CRL-1746) ST cells (ATCC CRL-1746)
Growth Conditions	Temperature: 37°C Incubation: 2-3 days at 37°C, a 5% in air atmosphere is recommended Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).

Effect on Host	Yes, in vitro effects: Cytopathic effects (fused and rounded cells) 18 to 24 hours post-inoculation CPE, fused and rounded cells 18-24 hours post-inculation

Name of Depositor	Iowa State Univ. Res. Fndn.
Special Collection	ATCC
Source	Culture of a novel TGEV-related Porcine Respiratory Coronavirus
References	Paul PS, et al. Pathogenic porcine respiratory coronavirus. US Patent 5,419,907 dated May 30 1995

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation

Porcine respiratory coronavirus (ATCC® VR-2384™)