Porcine respiratory coronavirus (ATCC® VR-2384)

Product Format: frozen

Agent Porcine respiratory coronavirus
Strain AR310
Applications
This strain provides a biological pure culture of a novel pathogenic porcine respiratory coronavirus (PRCV) and a vaccine derived therefrom effective against PRCV infection and transmissible gastroenteritis virus (TGEV) infection.
The virus produces moderate pneumonia in pigs, there is no indication of human health hazard.
Biosafety Level 2
Isolation
Culture of a novel TGEV-related Porcine Respiratory Coronavirus
Product Format frozen
Storage Conditions -70°C or colder
Comments
This strain provides a biological pure culture of a novel pathogenic porcine respiratory coronavirus (PRCV) and a vaccine derived therefrom effective against PRCV infection and transmissible gastroenteritis virus (TGEV) infection.
The virus produces moderate pneumonia in pigs, there is no indication of human health hazard.
Effect on Host
Yes, in vitro effects: Cytopathic effects (fused and rounded cells) 18 to 24 hours post-inoculation
CPE, fused and rounded cells 18-24 hours post-inculation
Recommended Host
swine testicular (ST) cells (ATCC CRL-1746)

Recommended Host:  Swine testicular cells (ATCC® CRL-1746™)

ST cells (ATCC CRL-1746)
Growth Conditions
Temperature: 37°C
Incubation:  2-3 days at 37°C, a 5% in air atmosphere is recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Effect on Host
Yes, in vitro effects: Cytopathic effects (fused and rounded cells) 18 to 24 hours post-inoculation
CPE, fused and rounded cells 18-24 hours post-inculation
Name of Depositor Iowa State Univ. Res. Fndn.
Special Collection ATCC
Source
Culture of a novel TGEV-related Porcine Respiratory Coronavirus
References

Paul PS, et al. Pathogenic porcine respiratory coronavirus. US Patent 5,419,907 dated May 30 1995