Avian coronavirus ATCC ® VR-21™

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Coronaviridae, Gammacoronavirus
Agent	Avian coronavirus
Strain	M41
Common Name	Infectious bronchitis virus (IBV)
Applications	Unlike fully egg-adapted strains that are non-immunogenic, this strain produces satisfactory immunity as determined by neutralization test for antibodies.
Biosafety Level	2
Product Format	frozen
Storage Conditions	-70°C or colder

Comments	The M41 (Massachusetts [Michigan State University Repository Code 41]) strain is the protype strain of IBV, however it should be noted that antigenicity and virulence of IBV change readily with passage in culture. Unlike fully egg-adapted strains that are non-immunogenic, this strain produces satisfactory immunity as determined by neutralization test for antibodies.
Effect on Host	Yes, in vivo effects: curling, stunting and pale coloring of infected embryos or chickens; may cause death.

Recommended Host	Production Host: Embryonated chicken egg (9 to 10-day old). Recommended Host: Embryonated chicken egg (9 to 10 day old) Alternate Host: Chicken (10-day old, intratracheal inoculation).
Growth Conditions	Temperature: 37.0°C Recommendations for Infection: Inoculate embryonated chicken eggs (9 to 10-day old) by the allantoic cavity route with 0.2 mL of a 1:10 to 1:100 dilution of virus in DPBS. Incubation: 2-6 days

Effect on Host	Yes, in vivo effects: curling, stunting and pale coloring of infected embryos or chickens; may cause death.

Name of Depositor	CH Cunningham, Michigan State University
Special Collection	ATCC
Chain of Custody	Deposited as Infectious bronchitis virus
Source	Isolated in 1941 from 19-day old chicks accidentally infected in the laboratory, University of Massachusetts.
References	Cunningham CH. Symposium on immunization against infectious bronchitis virus. I. Some basic properties of infectious bronchitis virus. Am. J. Vet. Res. 18: 648, 1957. PubMed: 13444588 Cunningham CH. . Adv. Vet. Sci. 14: 106-148, 1970. McIntosh K. . Curr. Top. Microbiol. Immunol. 63: 86-119, 1974. Cunningham CH. . Am. J. Vet. Res. 21: 498, 1960.

Permits

These permits may be required for shipping this product:

Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation

Avian coronavirus (ATCC® VR-21™)