Feline infectious peritonitis virus (ATCC® VR-2004)

Classification: Coronaviridae, Alphacoronavirus, Alphacoronavirus 1  /  Product Format: frozen 1.0 mL

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Classification Coronaviridae, Alphacoronavirus, Alphacoronavirus 1
Agent Feline infectious peritonitis virus
Strain DF2
Biosafety Level 2
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-2004 images
Comments Cross reacts with canine coronavirus, TGE and human 229B.
Effect on Host
CPE, Cell rounding and degeneration
Recommended Host
CRFK cells (ATCC® CCL-94™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 3-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, Cell rounding and degeneration
Name of Depositor EV Davis
Special Collection ATCC
Source Spleen of cat with typical FIP disease
References

Evermann JF, et al. Characterization of a feline infectious peritonitis virus isolate. Vet. Pathol. 18: 256-265, 1981. PubMed: 7467085

McKeirnan AJ et al. Comparative properties of feline coronaviruses in vitro. Can. J. Vet. Res. 51(2): 212-216, 1987. PubMed: 1255305

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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