Betacoronavirus 1 (ATCC® VR-1558™)

Classification: Coronaviridae, Betacoronavirus  /  Product Format: frozen 1 mL per vial

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Coronaviridae, Betacoronavirus
Agent Betacoronavirus 1
Strain OC43
Common Name Human coronavirus OC43
Applications
Respiratory research
Positive for Mycoplasma Yes
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
No antigenic cross reaction with Human coronavirus 229E (ATCC® VR-740™). VR-1558 is a TC-adapted item derived from virus passaged in mice (ATCC® VR-759™). Cleaned of mycoplasma with MRA.
Effect on Host
CPE, vacuolization of cytoplasm and sloughing
Recommended Host
HCT-8 [HRT-18] (ATCC® CCL-244™)
Growth Conditions
Temperature: 33°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere while rocking. End adsorption by adding virus growth medium.
Incubation: 4-6 days
Effect on Host
CPE, vacuolization of cytoplasm and sloughing
Name of Depositor JO Fleming
Special Collection ATCC
Chain of Custody
ATCC <-- JO Fleming <-- MK Cooney <-- AZ Kapikian
Source Man with cold-like illness
References

Bruckova M, et al. The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers. Proc. Soc. Exp. Biol. Med. 135: 431-435, 1970. PubMed: 4991398

McIntosh K, et al. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. USA 58: 2268-2273, 1967. PubMed: 4298953

McIntosh K, et al. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natl. Acad. Sci. USA 57: 933-940, 1967. PubMed: 5231356

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS