pLOGAN (ATCC® PVMC-8)

Organism: Beet curly top virus  /  Clone Type: Clone  /  Depositors: DC Stenger

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Designations pLOGAN
Species Beet curly top virus
Depositors DC Stenger
Applications
contains sequence
Plant research
Vector
Construct size (kb): 5.73

Construction of the clone:  Plasmid constructed by inserting 3038 bp Sal I linearized viral dsDNA into Sal I site of pUC8vector.

Insert
DNA: cDNA
Insert lengths(kb): 3.04
Tissue: Beet curly top virus
Insert Size (kb) 3.038
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Plasmid constructed by inserting 3038 bp SalI linearized viral dsDNA into SalI site of pUC8 vector. >95% sequence identity wiith BCTV- California. The clone is infectious. The ATCC sample contains 30ug of purifed plasmid DNA in TE buffer.
Classification Curtovirus
References

Stenger DC, et al. Genomic characterization of phenotypic variants of beet curly top virus. J. Gen. Virol. 71: 2211-2215, 1990. PubMed: 2230726

Stenger DC. Complete nucleotide sequence of the hypervirulent CFH strain of beet curly top virus. Mol. Plant-Microbe Interact. 7: 154-157, 1994. PubMed: 8167369

Stenger DC, et al. Phylogenetic relationships within the family Potyvirdae: wheat streak mosaic virus and brome streak mosaic virus are not members of the genus Rymovirus. Phytopathology 88: 782-787, 1998.

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