pWORLAND (ATCC® PVMC-7)

Organism: Beet curly top virus  /  Clone Type: Clone  /  Depositors: DC Stenger

Permits and Restrictions

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Designations pWORLAND
Species Beet curly top virus
Depositors DC Stenger
Applications
contains sequence
Plant research
Vector
Construct size (kb): 5.59499979019165

Construction of the clone:  Plasmid constructed by

inserting 2930 bp BamHI linearized viral dsDNA into BamHI site of pUC8vector.

Insert
DNA: cDNA
Insert lengths(kb): 2.930000066757202
Tissue: beet mild curly top virus
Insert Size (kb) 2.930
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Plasmid constructed by inserting 2930 bp BamHI linearized viral dsDNA into BamHI site of pUC8 vector. The clone is infectious. ATCC sample contains 50ug of purified plasmid DNA in 50ul of TE buffer.
Classification Curtovirus
References

Stenger DC, et al. Genomic characterization of phenotypic variants of beet curly top virus. J. Gen. Virol. 71: 2211-2215, 1990. PubMed: 2230726

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