pSP98 (ATCC® PVMC-25)

Organism: tomato yellow leaf curl bigeminivirus  /  Clone Type: Clone  /  Depositors: E Moriones

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Designations pSP98
GenBank Number

Z25751

Species tomato yellow leaf curl bigeminivirus
Depositors E Moriones
Applications
contains sequence
Plant research
Vector
Construct size (kb): 15.0

Construction of the clone:  A 1.8mer partial tandem repeat of the genome of M-TYLCV-Sar into binary vector pBin19.

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Bin 19
Intact vector size: 10.000
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: BamHI EcoRI HindIII
Polylinker sites: BamHI EcoRI HindIII SmaI SalI KpnI XbaI SstI
Construction: pRK252, pTiT7, pTiT37
Host range: Agrobacterium tumefaciens; higher plants; Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): kanR
replicon: RK2
Cross references:
Insert
DNA: genomic
Insert lengths(kb): 2.700000047683716
Insert Size (kb) 2.700
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Classification Geminivirus
References

Noris E, et al. High similarity among the tomato yellow leaf curl virus isolates from the west Mediterranean basin: the nucleotide sequence of an infectious clone from Spain. Arch. Virol. 135: 165-170, 1994. PubMed: 8198442

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