pBSW285-3/4 (ATCC® PVMC-15)

Organism: sweet potato leaf curl geminivirus  /  Clone Type: Clone  /  Depositors: RA Valverde

Permits and Restrictions

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Designations pBSW285-3/4
Species sweet potato leaf curl geminivirus
Depositors RA Valverde
Applications
contains sequence
Plant research
Vector
Construct size (kb): 5.5

Construction of the clone: PCR amplified fragment linked to pSt1 linkers and inserted into Pst1 digested pBS II SK.

Insert
DNA: cDNA
Insert lengths(kb): 2.539000034332275
Tissue: sweet potato leaf curl virus
Gene product: [pBSW285-3/4]
Insert Size (kb) 2.539
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
This clone contains reading frames of AV1, AV2, AC2, and AC3 of the viral genome.
Vials contain 50 ug of purified DNA in 50 ul of TE buffer.
PCR amplified fragment linked to pST1 linkers and inserted into Pst1 digested pBSIISK.
Classification Geminivirus
References

Lotrakul P, Valverde RA. Cloning of a DNA-A-like genomic component of sweet potato leaf curl virus: nucleotide sequence and phylogenetic relationships. Mol. Plant Pathol. : http://www.bspp.org.uk/mppol/1999/0422LOTRAKUL, 1999.

Rodrigo A Valverde, personal communication

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