pOT2 (ATCC® PVMC-1)

Organism: Odontoglossum ringspot virus  /  Clone Type: Clone  /  Depositors: SM Wong

Permits and Restrictions

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Designations pOT2
Species Odontoglossum ringspot virus
Depositors SM Wong
Applications
contains sequence
Plant research
Vector
Construct size (kb): 6.0

Plasmid constructed by inserting full-length cDNA clone of ORSV into pBlueScript  plasmid

Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: Cattleya odontoglossum ringspot
Disease: odontoglossum ringspot
Gene product: [ORSV clone]
Insert Size (kb) 3.0
Biosafety Level 1
Comments
Highly infectious in vitro RNA transcripts can be obtained using Promega's In Vitro Transcription kit (T7 RNA polymerase).
Plasmid constructed by inserting full-length cDNA clone of odontoglossum ringspot tobamovirus (ORSV) into pBluescript KS+ vector.
Classification Tobamovirus
References

Yu HH, Wong SM. A DNA clone encoding the full-length infectious genome of odontoglossum ringspot tobamovirus and mutagenesis of its coat protein gene. Arch. Virol. 143: 163-171, 1998. PubMed: 9505974

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