potato virus Y (ATCC® PVAS-766)

Classification: Potyvirus  /  Product Format: frozen

Classification Potyvirus
Agent potato virus Y
Strain potyvirus monoclonal antiserum [PTY10 cross-reactive monoclonal antiserum]
Applications
Test method: ACP-ELISA (Reacts in ACP-ELISA, dot-blot and Western-blot assays).
Reacts best with coat protein sub-unit rather than intact virus.
Plant research
Biosafety Level 1
Product Format frozen
Comments
Test animal: mouse
Test method: ACP-ELISA (Reacts in ACP-ELISA, dot-blot and Western-blot assays).
Hybridoma: 27G8H11
PTY 10 recognizes a potyvirus group-common, non-virion surface-located, conformation-independent, trypsin-resistant-core protein epitope found on at least 18 (of 30 tested) potyviruses.
Reacts best with coat protein sub-unit rather than intact virus.
Viruses tested, but reactivity not necessarily limited to: potato virus Y, pepper mottle, tobacco etch, tobacco vein mottling, turnip mosaic (7 of 8 strains), tulip breaking, sugarcane mosaic.
Viruses tested, continued: asparagus virus 1, cowpea aphid-borne mosaic, sweet potato latent, watermelon mosaic virus 2, papaya ringspot virus-W, zucchini mosaic, plum pox.
Name of Depositor R Jordan
References

Hammond J, Hammond RW. Molecular cloning, sequencing, and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene. J. Gen. Virol. 70: 1961-1974, 1989. PubMed: 2671258

McLaughlin MR, et al. Enzyme-linked immunosorbent assay of viruses infecting forage legumes. Phytopathology 74: 965-969, 1984.

Vance VB, et al. The complete nucleotide sequence of pepper mottle virus genomic RNA: comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191: 19-30, 1992. PubMed: 1413501

Hammond J, Lawson RH. An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissue. J. Virol. Methods 20: 203-217, 1988. PubMed: 3410929

Uyeda I, et al. Relatedness of the nucleotide sequence of the 3'-terminal region of clover yellow vein potyvirus RNA to bean yellow mosaic potyvirus RNA. Intervirology 32: 234-245, 1991. PubMed: 1864719

. Comparative potyvirus host range, serology, and coat protein peptide profiles of white lupin mosaic virus. Phytopathology 82: 566-571, 1992.

Hammond J, et al. Use of polyclonal antisera and monoclonal antibodies to examine serological relationships among three filamentous viruses of sweet potato. Phytopathology 82: 713-717, 1992.