Tobacco mosaic virus (ATCC® PVAS-135C-CO)

Classification: Tobamovirus  /  Product Format: frozen

Classification Tobamovirus
Agent Tobacco mosaic virus
Strain Rabbit antiserum to Tobacco mosaic virus, alkaline phosphatase conjugated
Applications
rabbit antiserum against purified tobacco mosaic virus
Quality control methods: ELISA
Plant research
Biosafety Level 1
Isolation
rabbit antiserum against purified tobacco mosaic virus
Source of antibody: rabbit serum
Purified IgG was conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
Product Format frozen
Comments
rabbit antiserum against purified tobacco mosaic virus
Specificity: tobacco mosaic virus
Immunogen: tobacco mosaic virus
Source of antibody: rabbit serum
Purification methods: affinity purification
Quality control methods: ELISA
The conjugate was developed by cross absorbing a rabbit polyclonal antiserum with healthy tobacco plant tissue followed by affinity purification of IgG.
Purified IgG was conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
Conjugated immunoglobulin is supplied in 1mM MgCl2, 15M NaCl and 50% glycerol and 0.05% sodium azide. Store at 4C after opening.
Name of Depositor ATCC
Special Collection ATCC
References

Meshi T, et al. Molecular cloning of the complementary DNA copies of the common and cowpea strains of tobacco mosaic virus RNA. Virology 118: 64-75, 1982.

Powell CA. Detection of three plant viruses by dot-immunobinding assay. Phytopathology 77: 306-309, 1987.

Fraile A, Garcia-Arenal F. A classification of the tobamoviruses based on comparisons among their 126K proteins. J. Gen. Virol. 71: 2223-2228, 1990. PubMed: 2230728

Hills GJ, et al. Immunogold localization of the intracellular sites of structural and nonstructural tobacco mosaic virus proteins. Virology 161: 488-496, 1987. PubMed: 3318095

Lehto K, et al. Insertion of sequences containing the coat protein subgenomic RNA promoter and leader in front of the tobacco mosaic virus 30K ORF delays its expression and causes defective cell-to-cell movement. Virology 174: 145-157, 1990. PubMed: 2294638

Garcia AF, et al. Strains and mutants of tobacco mosaic virus are both found in virus derived from single-lesion-passaged inoculum. Virology 132: 131-137, 1984.

Hegg LA, et al. Recognition of the tRNA-like structure in tobacco mosaic viral RNA by ATP/CTP:tRNA nucleotidyltransferases from Escherichia coli and Saccharomyces cerevisiae. J. Biol. Chem. 265: 17441-17445, 1990. PubMed: 2211637

Gallie DR, et al. Functional analysis of the tobacco mosaic virus tRNA-like structure in cytoplasmic gene regulation. Nucleic Acids Res. 19: 5031-5036, 1991. PubMed: 1923770