Influenza B virus (ATCC® VR-523)

Classification: Orthomyxoviridae, Influenzavirus B  /  Product Format: frozen

Classification Orthomyxoviridae, Influenzavirus B
Agent Influenza B virus
Strain B/Mass/3/66
Applications
Influenza Research
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder
Comments Please see ATCC Virology Guide for more detailed information on propagation
Effect on Host
Hemagglutinates chicken red blood cells and possible death of chicken embryo
Recommended Host
10 day old incubated specific pathogen free embryonated chicken eggs
Growth Conditions Temperature: 33°C
Recommendations for Infection: Dilute virus with PBS prior to inoculation. While candling the eggs, draw a pencil line around the air sac. Using an 18.5 gauge needle, poke a hole into the air sac. Using a 1.0 mL syringe fitted with a 22.5 gauge needle, insert the needle through the hole and into the allantoic cavity. Inject 0.2 mL of diluted inoculum into the allantoic cavity. Seal the hole with nail polish or sterile tape. Incubate eggs at 33°C 2 days, checking embryo viability every 12-18 hours and discard non-viable eggs. To harvest allantoic fluid, refrigerate eggs for at least 2 hours post incubation. Use sterile scissors to cut away the shell around the air sack. Aspirate the allantoic fluid with a syringe or a pipette. Avoid capturing blood or yolk in the allantoic fluid.
Incubation: 2 days with humidity
Effect on Host
Hemagglutinates chicken red blood cells and possible death of chicken embryo
Name of Depositor R.I.T. - Recherche et Industrie Therapeutiques
Source Throat washings from patient in US, 1966
Year of Origin 1966
References

. . NCDC Influenza-Respiratory Disease Surveillance Report 83: 4, 1967.