Recommendations for Infection: Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI 0.1. Adsorb for 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2 to 5 days at 35°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.