Influenza B virus (ATCC® VR-1735™)

Classification: Orthomyxoviridae, Influenzavirus B  /  Product Format: frozen 1 mL per vial

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Orthomyxoviridae, Influenzavirus B
Agent Influenza B virus
Strain B/Taiwan/2/62 (TC-adapted)
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1735 images
Comments
Derived by adaptation of egg-passage B/Taiwan/2/62 (ATCC VR-295) to MDCK (ATCC CCL-34) cells at ATCC. (Note: ATCC VR-295 and ATCC VR-1735 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).
Effect on Host
CPE, cell rounding, degeneration, sloughing
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 33°C
Recommendations For Infection: For best results, plate cells 24 to 48 hours in advance. Infect at 80-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33°C in a humidified, 5% CO2 atmosphere for 2 to 4 days, until CPE are well advanced in 100% of the culture. In TC, ATCC VR-1735 should be grown in serum free media that contains 1.0 µg/mL TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Incubation: 2-4 days
Effect on Host
CPE, cell rounding, degeneration, sloughing
Name of Depositor A.P. Kendal and ATCC
Special Collection ATCC
Source Patient in Taiwan, 1962
References

Green IJ, et al. The isolation and characterization of a new Influenza Type B virus on Taiwan. Am. J. Hyg. 79: 107-112, 1964. PubMed: 14114349

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Micrograph of Host Effects