Influenza A virus (H1N1) (ATCC® VR-1683)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen

Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H1N1)
Strain A/Swine/Iowa/15/30 (TC-adapted)
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2
Isolation
Derived by adaptation of egg-passage A/Swine/Iowa/15/30 (ATCC VR-333) to MDCK (ATCC CCL-34) cells. (Note: ATCC VR-333 and ATCC VR-1683 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).
Product Format frozen
Storage Conditions -70°C or colder
Comments
Derived by adaptation of egg-passage A/Swine/Iowa/15/30 (ATCC VR-333) to MDCK (ATCC CCL-34) cells. (Note: ATCC VR-333 and ATCC VR-1683 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).
Effect on Host
CPE (cell rounding, degeneration, sloughing)
CPE (cell rounding, degeneration, sloughing)
Growth Medium ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Recommended Host
Production Host: MDCK (ATCC CCL-34)
Alternate Hosts: Chicken embryo, MDCK and Monkey kidney cells (MkK)

Recommended Host:  MDCK (ATCC® CCL-34™)

Growth Conditions
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.5°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCC VR-1683 should be grown in serum free media that contains 1 ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Effect on Host
CPE (cell rounding, degeneration, sloughing)
CPE (cell rounding, degeneration, sloughing)
Name of Depositor A. P. Kendal and ATCC
Source
Swine in Iowa, 1930
References

Shope RE. Swine influenza : i. experimental transmission and pathology. J. Exp. Med. 54: 349-359, 1931. PubMed: 19869922