Influenza B virus (ATCC® VR-1535)

Classification: Orthomyxoviridae, Influenzavirus B  /  Product Format: frozen 1 mL per vial

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Classification Orthomyxoviridae, Influenzavirus B
Agent Influenza B virus
Strain B/Lee/40
Applications
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1535
Comments Derived by adaptation of egg allantoic fluid product ATCC VR-101 to MDCK cells (ATCC CCL-34). ATCC VR-101 and ATCC VR-1535 have not been compared for sequence or infectivity in eggs and tissue culture.
Effect on Host
CPE, rounding and sloughing
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
Effect on Host
CPE, rounding and sloughing
Name of Depositor MT Coleman, Centers for Disease Control and Prevention
Special Collection NCRR Contract
Source Throat washings from an influenza patient, Irvington, New York, 1940
References

Andrewes CH, et al. A short description of the Myxovirus group (Influenza and related viruses). Virology 1: 176-184, 1955. PubMed: 13267985

Francis T Jr. A new type of virus from epidemic influenza. Science 92: 405-408, 1940. PubMed: 17794275

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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