Influenza A virus (H1N1) (ATCC® VR-1520)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

View Permits

Classification Orthomyxoviridae, Influenzavirus A
Deposited As Influenza A virus
Agent Influenza A virus (H1N1)
Strain A/WS/33
Applications
Influenza Research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1520
Comments
Derived by adaptation of egg allantoic fluid product ATCC® VR-825™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-825™ and ATCC® VR-1520™ have not been compared for sequence or infectivity in eggs and tissue culture. Demonstrates cross-reactivity with Influenza A virus, A/PR/8/34 and swine influenza.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor WA Chappell, Centers for Disease Control and Prevention; ATCC
Special Collection NCRR Contract
Source Throat washings from an influenza patient
References

Burnet FM. A genetic approach to variation in influenza viruses; the characters of three substrains of influenza virus A (WS). J. Gen. Microbiol. 5: 46-53, 1951. PubMed: 14824469

Smith W, et al. A virus isolated from influenza patients. Lancet 2: 66-68, 1933.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation