Influenza A virus (H1N1) (ATCC® VR-1469™)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H1N1)
Strain A/PR/8/34
Applications
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1469 images
Comments
VR-1469 is a TC-adapted item derived from virus passaged in eggs (ATCC VR-95)
Effect on Host
CPE, cell rounding, degeneration, sloughing
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI. Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, cell rounding, degeneration, sloughing
Name of Depositor ATCC®, M Coleman, Centers for Disease Control and Prevention
Special Collection NCRR Contract
Source Patient in Puerto Rico, 1934
References

Francis T. Immunological relationships of strains of filterable virus recovered from cases of human influenza. Proc. Soc. Exp. Biol. Med. 32: 1172-1175, 1935.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Micrograph of Host Effects