pR16.11 purified plasmid DNA (ATCC® VRMC-8)

Organism: HRV type 16 molecular clone  /  Clone Type: Clone  /  Depositors: W Lee

Permits and Restrictions

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Designations pR16.11 purified plasmid DNA
GenBank Number

L24917

Species HRV type 16 molecular clone
Depositors W Lee
Applications
Contains full-length sequence
Vector Construct Size (Vector + Insert) (kB): 9.460                                                        
Host required: E. coli MV1193
Host medium: LB broth with 50 µg/mL ampicillin (ATCC Medium #1227)
Type of vectorE. coli plasmid
Name of vector:  pUC19 + T7 promoter
Vector size (kb):  nucleotides 1 to 400 and 2678 to 2686 were deleted in the cloning process.
Markers (ie. AMPR):  AMPR
Cloning site:  BamHI
Insert Insert: Human rhinovirus (HRV) type 16 cDNA
ClassificationPicornavirus, Rhinovirus, Human
Insert description (full-length, etc.):  full-length HRV 16 cDNA
Insert size (kb):  7.124 kb + poly A tail
Full/partial sequence available (ie. GenBank #): L24917, with differences noted (J. Virology 77: 6235-6244, 2003)

Markers (genes and/or restriction sites):
Enzyme  # sites  Fragment Length  Location of Cuts
BamH1        2          6455; 3005         
Pst1             3     6298; 2365; 527
Kpn1           3     4452; 4002; 1006
BgIII           4    5085;2045;1864;466
Insert Size (kb) 7.124 kb + poly A tail
Biosafety Level 2
Shipping Information 200 ng dried plasmid DNA
Comments

This full-length infectious clone is not published yet although the sequence was published (Virus Gene 9:177-181, 1995).


The first base of the HRV 16 genome was ligated to the last base of the T7 promoter. The HRV 16 genome has a poly (A) track of 52 A's at the 3' end. 


The full-length T7 transcripts of HRV 16 generated from this construct had a specific infectivity of about 5 x 106 PFU/µg, which is similar to the virion RNA.


The complete insert was found to differ from the GenBank sequence L24917 as follows (position in VRMC-8 sequence and base): 715 C, 2266 G, 2267 G, 2393 G, 2419 G, 2620 G, 2795 A, 3324 A, 3640 C, 3652 C, 4678 A, 4878 G, 4914 A, 5341 G, 6150 G, 6394 T, 6457 A, 6511 A. RefLee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280

References

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441

Lee WM, et al. Complete sequence of the RNA genome of human rhinovirus 16, a clinically useful common cold virus belonging to the ICAM-1 receptor group. Virus Genes 9: 177-181, 1995. PubMed: 7732663

Lee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280

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