pSVL(D3) (ATCC® 45137)

Organism: Hepatitis delta virus, Hepatitis delta virus  /  Clone Type: Clone  /  Depositors: JM Taylor

Permits and Restrictions

View Permits

Designations pSVL(D3)
GenBank Number

M21012

Species Hepatitis delta virus, Hepatitis delta virus
Depositors JM Taylor
Applications
Blood borne research
Sexually Transmitted Disease Research
Vector
Construct size (kb): 9.800000190734863
Insert
DNA: cDNA
Insert lengths(kb): 5.099999904632568
Tissue: DNA from liver of an infected woodchuck
Gene product: delta antigen
Target Gene: delta antigen
Insert Size (kb) 5.100
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information

Shipped:  in Escherichia coli containing the plasmid.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.7, 2.1, 1.65 (triplet); EcoRI--2.7, 2.1, 1.65 (triplet); PstI--4.0, 2.4, 1.3 (doublet), 0.5; BglI--2.5, 1.65 (doublet), 1.5, 1.4, 1.1; BglII--6.5, 1.65 (doublet).
This is plasmid in E.coli that can be used to transfect COS-7 cells.
The unit genome contains the following restriction sites (bp from the 5' end): BglI--716; BglII--224; EcoRI--1427; HincII--962, 1200; NcoI--576; PstI--651, 1083; SalI--962; SmaI--479, 1107; XbaI--781; XhoI--1272, 1506, 1545.
Virus DNA cloned after passage in liver of woodchuck
The insert consists of a head to tail trimer of the full length genome (1.679 kb) beginning at the unique EcoRI site and oriented so that transcription from the SV40 late promoter gives genomic RNA.
Excising with BamHI+EcoRI yields 3 insert fragments of 1.7 kb and vector fragments of 1.8 kb and 3.0 kb. The construct has a unique BamHI site.
Transfected COS-7 cells replicate the HDV RNA genome, and produce the 195 aa small form of the delta antigen, encoded from nt 1598 to 1014.
References

Kuo MY, et al. Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen. J. Virol. 63: 1945-1950, 1989. PubMed: 2649689

Kuo MY, et al. Molecular cloning of hepatitis delta virus RNA from an infected woodchuck liver: sequence, structure, and applications. J. Virol. 62: 1855-1861, 1988. PubMed: 3367426

John M Taylor, personal communication

Product Sheet
Product Sheet
Product Sheet