Chlamydia trachomatis (ATCC® VR-902B)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

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Classification Chlamydiaceae, Chlamydia
Agent Chlamydia trachomatis
Strain Lymphogranuloma venereum (LGV II) strain 434
Applications
Sexually Transmitted Disease Research
Biosafety Level 3
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

LGV II strain 434 was initially accessioned as an egg passaged preparation (VR-902) and subsequently adapted to cell culture at ATCC and reaccessioned as VR-902B. Dilute high titer stocks to avoid cell toxicity.
Effect on Host
Inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies.
Recommended Host
McCoy cells (ATCC CRL-1696)
Growth Conditions
Temperature: 36°C to 37°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended.
Effect on Host
Inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies.
Name of Depositor J Schachter, adapted at ATCC
Source Bubo from human with LGV
References

Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618

Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529

Cross References

Nucleotide (GenBank) : U68443 Chlamydia trachomatis strain L2/434/BU 16S and 23S ribosomal RNA operon, complete sequence.

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