West Nile virus (ATCC® VR-1621)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen Each vial of VR-1621 contains approximately 100 µL of purified monoclonal antibody in PBS.

Classification Flaviviridae, Flavivirus
Agent West Nile virus
Strain Monoclonal antibody to West Nile virus Nonstructural Protein 1, Clone 22-NS1 (produced in vitro)
Common Name West Nile virus
Applications
Mouse monoclonal antibody prepared against the nonstructural protein 1 (NS1) of West Nile virus (WNV) was purified from clone 22-NS1 hybridoma supernatant by protein G affinity chromatography.
The antibody is reported to be reactive using flow cytometry and Western blot analysis.
Emerging infectious disease research
Biosafety Level 1
Isolation
Mouse monoclonal antibody prepared against the nonstructural protein 1 (NS1) of West Nile virus (WNV) was purified from clone 22-NS1 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone 22-NS1 antibody is reported to bind to domain II-III in the NS1 glycoprotein.
Product Format frozen Each vial of VR-1621 contains approximately 100 µL of purified monoclonal antibody in PBS.
Storage Conditions  Store at -20°C or colder immediately upon arrival Repeated freeze-thaw cycles should be avoided
Comments
Antibody Class: IgG2aκ
Mouse monoclonal antibody prepared against the nonstructural protein 1 (NS1) of West Nile virus (WNV) was purified from clone 22-NS1 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone 22-NS1 antibody is reported to bind to domain II-III in the NS1 glycoprotein.
Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC CCL-81) infected with WNV and by ELISA using WNV-infected cell lysates [WNV, Eg101 (Egypt 1951)]. The antibody is reported to be reactive using flow cytometry and Western blot analysis.
Effect on Host
Mouse monoclonal antibody prepared against the nonstructural protein 1 (NS1) of West Nile virus (WNV) was purified from clone 22-NS1 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone 22-NS1 antibody is reported to bind to domain II-III in the NS1 glycoprotein.
Effect on Host
Mouse monoclonal antibody prepared against the nonstructural protein 1 (NS1) of West Nile virus (WNV) was purified from clone 22-NS1 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone 22-NS1 antibody is reported to bind to domain II-III in the NS1 glycoprotein.
Name of Depositor MS Diamond
References

Chung KM, et al. Antibodies against West Nile Virus Nonstructural Protein NS1 Prevent Lethal Infection through Fc γ Receptor-Dependent and -Independent Mechanisms. J. Virol. 80: 1340-1351, 2006. PubMed: 16415011.

Chung KM, et al. Antibody Recognition of Cell Surface-Associated NS1 Triggers Fc-γ Receptor-Mediated Phagocytosis and Clearance of West Nile Virus-Infected Cells. J. Virol. 81: 9551-9555, 2007. PubMed: 17582005.