West Nile virus (ATCC® VR-1617™)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen Each vial of VR-1617 contains approximately 100 µL of purified monoclonal antibody in PBS.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Flaviviridae, Flavivirus
Agent West Nile virus
Strain Monoclonal antibody to West Nile virus Envelope Protein, Clone E114 (produced in vitro)
Common Name West Nile virus
Applications
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E114 hybridoma supernatant by protein G affinity chromatography.
The antibody is reported to be non-neutralizing and reactive using flow cytometry and Western blot analysis.
Emerging infectious disease research
Biosafety Level 1
Isolation
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E114 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E114 antibody is reported to bind to domain III in the envelope glycoprotein.
Product Format frozen Each vial of VR-1617 contains approximately 100 µL of purified monoclonal antibody in PBS.
Storage Conditions  Store at -20°C or colder immediately upon arrival Repeated freeze-thaw cycles should be avoided
Comments
Antibody Class: IgG1κ
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E114 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E114 antibody is reported to bind to domain III in the envelope glycoprotein.
Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC CCL-81) infected with WNV and by ELISA using WNV-infected cell lysates [WNV, Eg101 (Egypt 1951)]. The antibody is reported to be non-neutralizing and reactive using flow cytometry and Western blot analysis.
Effect on Host
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E114 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E114 antibody is reported to bind to domain III in the envelope glycoprotein.
Effect on Host
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E114 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E114 antibody is reported to bind to domain III in the envelope glycoprotein.
Name of Depositor MS Diamond
References

Oliphant T, et al. Antibody Recognition and Neutralization Determinants on Domains I and II of West Nile Virus Envelope Protein. J. Virol. 80:12149-12159, 2006. PubMed:17035317.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS