California encephalitis virus (ATCC® VR-761)

Classification: Bunyaviridae, Orthobunyavirus  /  Product Format: freeze-dried

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Classification Bunyaviridae, Orthobunyavirus
Agent California encephalitis virus
Strain TRVL 9375
Common Name Melao virus
Applications
HA produced in goose RBC at pH 6.0 to pH 7.0 at 4, 22, and 37°C with optimal temperature of 22°C.
Biosafety Level 2
Product Format freeze-dried
Storage Conditions  -70°C or colder
Comments
Sensitive to sodium deoxycholate. HA produced in goose RBC at pH 6.0 to pH 7.0 at 4, 22, and 37°C with optimal temperature of 22°C.
Officially recognized as prototype strain by American Committee on Arthropod-borne Viruses.
Effect on Host
In vivo: Death of host animal
Death
Recommended Host
Suckling mouse

Production Host:  Suckling mouse

Growth Conditions
Recommendations for Infection: Inoculate intracerebrally into suckling mice. Resuspend 10% sMb with 7.5% BSA in PBS.
Incubation: 2-3 days
Effect on Host
In vivo: Death of host animal
Death
Name of Depositor RE Shope
Special Collection ATCC
Chain of Custody
Deposited as Melao virus
Source
Mosquito pool, Aedes (Ochlerotatus) scapularis, Trinidad, 1955
References

Anderson CR, et al. Melao virus, a new agent isolated from Trinidadian mosquitoes. Am. J. Trop. Med. Hyg. 11: 687-690, 1962. PubMed: 13990007

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