Human adenovirus 35 (ATCC® VR-718)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus B  /  Product Format: frozen

Classification Adenoviridae, Mastadenovirus, Human adenovirus B
Agent Human adenovirus 35
Strain Holden
Applications
Respiratory research
Biosafety Level 2
Isolation
Lung and kidney tissues from 61-year-old woman who died with diffuse interstitial pneumonia
Product Format frozen
Storage Conditions -70°C or colder
Comments
Acid and chloroform stable, partially heat-labile, no cationic stabilization. Group CF (hexon) titers of 1:16-1:128 expected.
Effect on Host
Yes, in vitro effects: Cytopathic effects (typical adenovirus CPE)
CPE, refractile rounding, clumping, and sloughing
Recommended Host
HEp-2 cells (ATCC CCL-23)

Recommended Host: HEp-2 (ATCC® CCL-23™)

WI-38 cells (ATCC CCL-75); human embryonic lung fibroblast; RU-1 cells (human embryonic lung); primary human amnion cells; primary human embryonic kidney cells; KB cells (ATCC CCL-17); HEp-2 cells (ATCC CCL-23); HeLa cells (ATCC CCL-2)
Growth Conditions
Temperature: 35.0°C
Duration: 4 days
Effect on Host
Yes, in vitro effects: Cytopathic effects (typical adenovirus CPE)
CPE, refractile rounding, clumping, and sloughing
Name of Depositor JC Hierholzer
Source
Lung and kidney tissues from 61-year-old woman who died with diffuse interstitial pneumonia
References

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Myerowitz RL, et al. Fatal disseminated adenovirus infection in a renal transplant recipient. Am. J. Med. 59: 591-598, 1975. PubMed: 170822

Stalder H, et al. New human adenovirus (candidate adenovirus type 35) causing fatal disseminated infection in a renal transplant recipient. J. Clin. Microbiol. 6: 257-265, 1977. PubMed: 71305

Cross References

Nucleotide (GenBank) : U52572 Human adenovirus type 35 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds.