Adeno-associated virus 2 (ATCC® VR-680)

Classification: Parvoviridae, Dependovirus, Adeno-associated virus-2  /  Product Format: frozen

Classification Parvoviridae, Dependovirus, Adeno-associated virus-2
Deposited As Adeno-associated virus type 2
Agent Adeno-associated virus 2
Strain H
Applications
Resistant to heat 56C 4 hours; this property can be used to selectively inactivate the helper adenovirus.
Cross-reacts with AAV-3; no hemagglutinin detected.
Biosafety Level 2
Isolation
Adenovirus type 12 strain 97838 in KB cells
Product Format frozen
Storage Conditions -70°C or colder
Comments
Resistant to heat 56C 4 hours; this property can be used to selectively inactivate the helper adenovirus. Resistant to ether and chloroform.
Cross-reacts with AAV-3; no hemagglutinin detected. Depends upon helper adenovirus for replication.
Effect on Host
Yes, in vitro effects: Cytopathic effects (typical adenovirus CPE)
Adenovirus CPE, refractile rounding and cell sloughing.  AAV shows no CPE.
Recommended Host
human embryonic kidney cells; KB cells (ATCC CCL-17); HeLa cells (ATCC CCL-2) with Adenovirus 2 helper

Recommended Host:  HEK-293 cells (ATCC® CRL-1573TM)

Growth Conditions
Temperature: 36.0°C
Duration: 2 days
Effect on Host
Yes, in vitro effects: Cytopathic effects (typical adenovirus CPE)
Adenovirus CPE, refractile rounding and cell sloughing.  AAV shows no CPE.
Name of Depositor MD Hoggan
Source
Adenovirus type 12 strain 97838 in KB cells
References

Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666